Permeability and gating properties of human connexins 26 and 30 expressed in HeLa cells.

@article{Beltramello2003PermeabilityAG,
  title={Permeability and gating properties of human connexins 26 and 30 expressed in HeLa cells.},
  author={M. Beltramello and Massimiliano Bicego and Valeria Piazza and Catalin Dacian Ciubotaru and Fabio Mammano and Paola D'andrea},
  journal={Biochemical and biophysical research communications},
  year={2003},
  volume={305 4},
  pages={1024-33}
}
Human connexins 26 and 30 were expressed either through the bicistronic pIRES-EGFP expression vector or as EYFP-tagged chimeras. When transiently transfected in communication-incompetent HeLa cells, hCx26-pIRES transfectants were permeable to dyes up to 622 Da, but were significantly less permeable to 759 Da molecules. Under the same conditions, permeability of hCx26-EYFP fusion products was comparable to that of hCx26-pIRES, but with significant increase in diffusion at 759 Da, possibly as a… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 35 references

Functional analysis of recessive mutations of human connexin 26 associated with nonsyndromic deafness

K. Jordan, A. Bukauskiene, +3 authors V. K. Verselis
FEBS Lett . • 2003

Roles of Met-34

A. Oshima, T. Doi, K. Mitsuoka, S. Maeda, Y. Fujiyoshi
Cys-64, and Arg-75 in the assembly of human connexin 26. Implication for key amino acid residues for channel formation and function, J. Biol. Chem. 278 • 2003

Functional studies of human skin disease- and deafness-associated connexin 30 mutations.

Biochemical and biophysical research communications • 2002
View 3 Excerpts

Hearing loss: frequency and functional studies of the most common connexin26 alleles.

Biochemical and biophysical research communications • 2002
View 4 Excerpts

Membrane targeting and voltage gating of connexin 26 and 30 fused to GFP colour variants

M. Beltramello, V. Piazza, F. Mammano
J. Physiol. 544.P • 2002
View 2 Excerpts

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