Peripheral effects induced in BALB/c mice infected with DENV by the intracerebral route.

Abstract

The lack of an immunocompetent animal model for dengue mimicking the disease in humans is a limitation for advances in this field. Inoculation by intracerebral route of neuroadapted dengue strains in mice is normally lethal and provides a straightforward readout parameter for vaccine testing. However, systemic effects of infection and the immune response elicited in this model remain poorly described. In the present work, BALB/c mice infected by the intracerebral route with neuroadapted DENV2 exhibited several evidences of systemic involvement. DENV-inoculated mice presented virus infective particles in the brain followed by viremia, especially in late stages of infection. Infection induced cellular and humoral responses, with presence of activated T cells in spleen and blood, lymphocyte infiltration and tissue damages in brain and liver, and an increase in serum levels of some pro-inflammatory cytokines. Data highlighted an interplay between the central nervous system commitment and peripheral effects under this experimental condition.

DOI: 10.1016/j.virol.2015.12.006

Cite this paper

@article{Oliveira2016PeripheralEI, title={Peripheral effects induced in BALB/c mice infected with DENV by the intracerebral route.}, author={Edson R. A. Oliveira and Juliana Fernandes Amorim and Marciano Viana Paes and Ant{\'o}nio Siborro Azevedo and Ant{\^o}nio J. S. Gonçalves and Simone Miranda da Costa and Marcio Mantuano-Barradas and Tiago Fajardo P{\'o}voa and Juliana de Meis and Carlos Alberto Bas{\'i}lio-de-Oliveira and Ana Cristina Martins de Almeida Nogueira and Ada M. B. Alves}, journal={Virology}, year={2016}, volume={489}, pages={95-107} }