Peripheral administration of κ-opioid receptor antagonist stimulates gonadotropin-releasing hormone pulse generator activity in ovariectomized, estrogen-treated female goats.

  title={Peripheral administration of $\kappa$-opioid receptor antagonist stimulates gonadotropin-releasing hormone pulse generator activity in ovariectomized, estrogen-treated female goats.},
  author={T. Sasaki and D. Ito and T. Sonoda and Y. Morita and Y. Wakabayashi and T. Yamamura and H. Okamura and S. Oishi and T. Noguchi and N. Fujii and Y. Uenoyama and H. Tsukamura and K. Maeda and F. Matsuda and S. Ohkura},
  journal={Domestic animal endocrinology},
Pulsatile gonadotropin-releasing hormone (GnRH) secretion is indispensable for reproduction in mammals. Kisspeptin neurons in the hypothalamic arcuate nucleus (ARC), referred to as KNDy neurons because of the coexpression of neurokinin B and dynorphin A, are considered as components of the GnRH pulse generator that produces rhythmic GnRH secretion. The present study aimed to investigate if peripheral administration of PF-4455242, a κ-opioid receptor (KOR, a dynorphin A receptor) antagonist… Expand
Facilitatory and inhibitory role of central amylin administration in the regulation of the gonadotropin-releasing hormone pulse generator activity in goats
The results suggest that central amylin-CTR signaling has a biphasic role in the regulation of GnRH pulse generator activity by acting on cells other than the arcuate kisspeptin neurons in goats. Expand
Paraventricular dynorphin A neurons mediate LH pulse suppression induced by hindbrain glucoprivation in female rats.
The results suggest that the PVN Dyn neurons, at least in part, mediate LH pulse suppression induced by the hindbrain or peripheral glucoprivation, and Dyn neurons may directly suppress the ARC kisspeptin neurons in female rats. Expand
Kobayashi Award 2019: The neuroendocrine regulation of the mammalian reproduction.
  • H. Tsukamura
  • Medicine
  • General and comparative endocrinology
  • 2021
The programming effect of estrogen on kisspeptin neurons in the developmental brain is discussed to uncover the mechanism underlying the sex difference in GnRH/gonadotropin release as well as an irreversible infertility induced by supra-physiological estrogen exposure in rodent models. Expand
Role of KNDy Neurons Expressing Kisspeptin, Neurokinin B, and Dynorphin A as a GnRH Pulse Generator Controlling Mammalian Reproduction
Increasing evidence accumulated during the past two decades has demonstrated that the then-novel kisspeptin, which was discovered in 2001, the known neuropeptides neurokinin B and dynorphin A, whichExpand
Kisspeptin: A Central Regulator of Reproduction in Mammals
The mechanism suppressing reproductive function during lactation and other stress conditions through inhibition of kisspeptin neurons and consequent GnRH/gonadotropin secretion is discussed, to provide insights on the possibility of new strategies to control reproductive performance in domestic farm animals. Expand
Unraveling the Neural Mechanisms Underlying the GnRH Pulse Generator: An Update
Abstract The discovery of episodic LH secretion in 1970 initiated considerable effort by reproductive neuroendocrinologists to understand the neural mechanisms underlying the synchronous release ofExpand
Establishment of immortalised cell lines derived from female Shiba goat KNDy and GnRH neurones
Goat KNDy and GnRH neurone cell lines are established that could be used to analyse molecular and cellular mechanisms regulating KNDY and Gn RH neurones in vitro, facilitating the clarification of reproductive neuroendocrine mechanisms in ruminants. Expand
Journal of Reproduction and Development
We examined whether the use of in vivo-matured oocytes, collected by ovum pick-up (OPU) from superstimulated Japanese Black cows, can improve the productivity and quality of in vitro producedExpand


Chronic Peripheral Administration of Kappa-Opioid Receptor Antagonist Advances Puberty Onset Associated with Acceleration of Pulsatile Luteinizing Hormone Secretion in Female Rats
The present results suggest that the inhibitory input of dynorphin-KOR signaling plays a role in the prepubertal restraint of GnRH/LH secretion in normal developing female rats and that attenuation of dynasty-kappa-opioid receptor (KOR) signaling and increase in NKB-NK3R signaling trigger the onset of puberty in female rats. Expand
Dynorphin-Kappa Opioid Receptor Signaling Partly Mediates Estrogen Negative Feedback Effect on LH Pulses in Female Rats
The present result suggests that Dyn-KOR signaling is a part of estrogen negative feedback action on GnRH/LH pulses by reducing the kisspeptin release in female rats. Expand
A neurokinin 3 receptor-selective agonist accelerates pulsatile luteinizing hormone secretion in lactating cattle†
It is suggested that senktide infusion elicits an increase in LH pulse frequency that may stimulate follicular development and, in turn, induce the first postpartum ovulation in lactating cattle. Expand
Regulation of Gonadotropin-Releasing Hormone Secretion by Kisspeptin/Dynorphin/Neurokinin B Neurons in the Arcuate Nucleus of the Mouse
A model whereby NKB and dynorphin act autosynaptically onkisspeptin neurons in the Arc to synchronize and shape the pulsatile secretion of kisspeptin and drive the release of GnRH from fibers in the median eminence is proposed. Expand
Kisspeptin, neurokinin B, and dynorphin act in the arcuate nucleus to control activity of the GnRH pulse generator in ewes.
It is concluded that the KNDy neuropeptides act in the arcuate nucleus to control episodic GnRH secretion in the ewe, but afferent input from GnRH neurons to this area does not, which raises the possibility that kisspeptin contributes to the control of GnRH pulse frequency. Expand
A possible role of neuropeptide Y as a mediator of undernutrition to the hypothalamic gonadotropin-releasing hormone pulse generator in goats.
The results demonstrate that the activity of the GnRH pulse generator is suppressed by fasting in the presence of E(2) and suggest that NPY acts as a mediator of undernutrition to the Gn RH pulse generator. Expand
Neurokinin B and Dynorphin A in Kisspeptin Neurons of the Arcuate Nucleus Participate in Generation of Periodic Oscillation of Neural Activity Driving Pulsatile Gonadotropin-Releasing Hormone Secretion in the Goat
Investigating the possible coexpression of kisspeptin, neurokinin B (NKB), and dynorphin A (Dyn) in neurons of the ARC of the goat and evaluating their potential roles in generating GnRH pulses found that all three neuropeptides are coexpressed in the same population of neurons. Expand
Ovarian and hormonal responses to single or continuous peripheral administration of senktide, a neurokinin 3 receptor agonist, during the follicular phase in goats.
It is suggested that continuous infusion of senktide in follicular phase goats can cause a sustained increase in LH and advance the time of ovulation. Expand
κ-Opioid Receptor Is Colocalized in GnRH and KNDy Cells in the Female Ovine and Rat Brain.
The findings suggest that the inhibitory effects of dynorphin on pulsatile GnRH secretion may occur either indirectly by actions upon KOR within the KNDy population and/or directly via the activation of KOR on GnRH cells. Expand
Neurobiological mechanisms underlying GnRH pulse generation by the hypothalamus
The present article provides a brief historical background to the work that led to the concept of the GnRH pulse generator, and proposes that kisspeptin neurons in the arcuate nucleus are key players in this regard. Expand