Periodate-acid treatment of sections permits on-grid immunogold localization of pea seed vicilin in ER and Golgi

@article{Craig2005PeriodateacidTO,
  title={Periodate-acid treatment of sections permits on-grid immunogold localization of pea seed vicilin in ER and Golgi},
  author={Stuart Craig and D. J. Goodchild},
  journal={Protoplasma},
  year={2005},
  volume={122},
  pages={35-44}
}
The legume seed reserve protein vicilin has been localized in developing pea cotyledons by immunogold labelling on sections of glutaraldehyde-osmium-fixed, resin-embedded tissue. By treating sections with periodate and acid before antibody labelling, a 20-fold increase in specific antibody binding was observed. The densest label occurred over vacuolar contents, previously identified as the site of protein storage, and over electron-dense cisternae occasionally seen to be continuous with the… CONTINUE READING
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