Perfusion of trout liver in situ. Description and validation of the technique.

  title={Perfusion of trout liver in situ. Description and validation of the technique.},
  author={A I Cascales and Francisca P{\'e}rez-Llamas and J Francisco Garc{\'i}a Mar{\'i}n and Salvador Zamora},
  journal={Reproduction, nutrition, development},
  volume={37 1},
The aim of this study was to develop a method for perfusing isolated trout livers that would make it possible to study hepatic metabolism in the whole organ. Rainbow trout (Oncorhynchus mykiss Walbaum) subjected to different fasting periods of 24, 48 or 96 h were used in all the experiments. A non-recirculating system was applied at a flow rate of 0.2 mL/min. The perfusion medium was a specific saline solution for salmonids oxygenated by a multi-bulb glass oxygenator. Viability assays included… CONTINUE READING

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