Perfusion and chemical monitoring of living cells on a microfluidic chip.

  title={Perfusion and chemical monitoring of living cells on a microfluidic chip.},
  author={Jonathan G. Shackman and Gabriella M. Dahlgren and Jennifer L Peters and Robert T Kennedy},
  journal={Lab on a chip},
  volume={5 1},
A microfluidic device that incorporates continuous perfusion and an on-line electrophoresis immunoassay was developed, characterized, and applied to monitoring insulin secretion from single islets of Langerhans. In the device, a cell chamber was perfused with cell culture media or a balanced salt solution at 0.6 to 1.5 microL min(-1). The flow was driven by gas pressure applied off-chip. Perfusate was continuously sampled at 2 nL min(-1) by electroosmosis through a separate channel on the chip… CONTINUE READING
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