Performance of two quantitative PCR methods for microbial source tracking of human sewage and implications for microbial risk assessment in recreational waters.

@article{Staley2012PerformanceOT,
  title={Performance of two quantitative PCR methods for microbial source tracking of human sewage and implications for microbial risk assessment in recreational waters.},
  author={Christopher Staley and Katrina V. Gordon and Mary Schoen and Valerie J. Harwood},
  journal={Applied and environmental microbiology},
  year={2012},
  volume={78 20},
  pages={
          7317-26
        }
}
Before new, rapid quantitative PCR (qPCR) methods for assessment of recreational water quality and microbial source tracking (MST) can be useful in a regulatory context, an understanding of the ability of the method to detect a DNA target (marker) when the contaminant source has been diluted in environmental waters is needed. This study determined the limits of detection and quantification of the human-associated Bacteroides sp. (HF183) and human polyomavirus (HPyV) qPCR methods for sewage… CONTINUE READING

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