Performance of serial time-encoded amplified microscopy

@article{Tsia2010PerformanceOS,
  title={Performance of serial time-encoded amplified microscopy},
  author={Kevin K. Tsia and Keisuke Goda and Dale L. Capewell and Bahram Jalali},
  journal={CLEO/QELS: 2010 Laser Science to Photonic Applications},
  year={2010},
  pages={1-2}
}
  • K. Tsia, K. Goda, B. Jalali
  • Published 10 May 2010
  • Biology
  • CLEO/QELS: 2010 Laser Science to Photonic Applications
Serial time-encoded amplified microscopy (STEAM) is a new high-sensitivity ultrafast real-time imaging modality. Here we describe an analysis of its spatial resolution, frame rate, and detection sensitivity. 
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108 Citations
Background Citations
8
Methods Citations
6

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108 Citations

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References

SHOWING 1-10 OF 21 REFERENCES
Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena
TLDR
This work maps a two-dimensional (2D) image into a serial time-domain data stream and simultaneously amplifies the image in the optical domain and overcomes the compromise between sensitivity and frame rate without resorting to cooling and high-intensity illumination.
Simultaneous mechanical-scan-free confocal microscopy and laser microsurgery.
TLDR
An endoscope-compatible single-fiber-based device that performs simultaneous confocal microscopy and high-precision laser microsurgery and holds promise for creating highly miniaturized endoscopes for applications such as brain tumor, pediatric, and endovascular surgeries where high- Precision, small, and flexible probes are required.
A GRISM-based probe for spectrally encoded confocal microscopy.
TLDR
A novel use of a single-optical-axis element based on high index-of-refraction prisms and a transmission holographic grating for the design of narrow diameter SECM devices is described.
Microscopy and its focal switch
TLDR
The principles of these methods together with their differences in implementation and operation are discussed, and potential developments are outlined.
Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
TLDR
3D stochastic optical reconstruction microscopy (STORM) is demonstrated by using optical astigmatism to determine both axial and lateral positions of individual fluorophores with nanometer accuracy, allowing the 3D morphology of nanoscopic cellular structures to be resolved.
Femtosecond real-time single-shot digitizer
TLDR
A single-shot Digitizer with a 10 Tsample/s sampling rate is demonstrated with a photonic time stretch preprocessor that slows down the electrical waveform by an unprecedented factor of 250 before it is captured by a commercial electronic digitizer.
Spectrally encoded miniature endoscopy.
TLDR
A method for performing miniature endoscopy with a high number of resolvable points is presented, using a broad-bandwidth light source and a diffraction grating to simultaneously detected the reflectivity at multiple points along a transverse line within the sample.
Amplified dispersive Fourier-transform imaging for ultrafast displacement sensing and barcode reading
Dispersive Fourier transformation is a powerful technique in which the spectrum of an optical pulse is mapped into a time-domain waveform using chromatic dispersion. It replaces a diffraction grating…
Amplified wavelength–time transformation for real-time spectroscopy
Real-time spectroscopy provides invaluable information about the evolution of dynamical processes, especially non-repetitive phenomena. Unfortunately, the continuous acquisition of rapidly varying…
Real-time spectroscopy with subgigahertz resolution using amplified dispersive Fourier transformation
Dispersive Fourier transformation is a powerful technique in which spectral information is mapped into the time domain using chromatic dispersion. It replaces a spectrometer with an electronic…
...
...