Performance of genomic bordering elements at predefined genomic loci.

  title={Performance of genomic bordering elements at predefined genomic loci.},
  author={Sandra Goetze and Alexandra S. Baer and Silke Winkelmann and Kristina Nehlsen and Jost Seibler and K. -E. Maass and J{\"u}rgen Bode},
  journal={Molecular and cellular biology},
  volume={25 6},
Eukaryotic DNA is organized into chromatin domains that regulate gene expression and chromosome behavior. Insulators and/or scaffold-matrix attachment regions (S/MARs) mark the boundaries of these chromatin domains where they delimit enhancing and silencing effects from the outside. By recombinase-mediated cassette exchange (RMCE), we were able to compare these two types of bordering elements at a number of predefined genomic loci. Flanking an expression vector with either S/MARs or two copies… CONTINUE READING