Performance of diamino fluorophores for the localization of sources and targets of nitric oxide.

  title={Performance of diamino fluorophores for the localization of sources and targets of nitric oxide.},
  author={Juan Rodriguez and Vicky Specian and Ronald E. Maloney and David Jourd’heuil and Martin Feelisch},
  journal={Free radical biology \& medicine},
  volume={38 3},
2-Amino-3′-dialkylaminobiphenyl-based fluorescent intracellular probes for nitric oxide surrogate N2O3†
A family of fluorescent probes based on a 2-amino-3′-dialkylaminobiphenyl core, which demonstrates similar or better sensitivity and higher selectivity for NO than DAF, making it an attractive potential alternative for NO tracking in various applications.
Use of Fluorescence Probes for Detection of Reactive Nitrogen Species: A Review
An account of the fluorescence probes that have been used for detection of nitric oxide, peroxynitrite anion, as well as of some of its derivatives in biological and nonbiological media is given.
Nitric oxide (NO) detection by DAF fluorescence and chemiluminescence: a comparison using abiotic and
Because of controversies in the literature on nitric oxide (NO) production by plants, NO detection by the frequently used diaminofluorescein (DAF-2 and DAF-2DA) and by chemiluminescence were compared
Nitric oxide (NO) detection by DAF fluorescence and chemiluminescence: a comparison using abiotic and biotic NO sources.
Changes in DAF fluorescence are not necessarily indicative for NO production, but may also reflect NO oxidation and/or production of other DAF-reactive compounds.
Detection of nitric oxide in single cells.
Three categories of analytical techniques enabling NO detection at single cell levels are highlighted: fluorescence microscopy, capillary electrophoresis with laser induced fluorescence detection, and electrochemistry, and the basic principles, performance, applications, figures of merits and limitations are presented.
(-)-Epicatechin elevates nitric oxide in endothelial cells via inhibition of NADPH oxidase.


Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins.
Fluorescence detection with visible light excitation and high sensitivity enabled the practical assay of NO production in living cells, and Membrane-permeable DAF-2 diacetate can be used for real-time bioimaging of NO with fine temporal and spatial resolution.
Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry.
The present novel diaminofluorescein fluorometry should be useful not only for sensitive NO assay, but also for NO imaging in a variety of biological specimens.
Detection of intracellular nitric oxide using a combination of aldehyde fixatives with 4,5-diaminofluorescein diacetate
Abstract. Using 4,5-diaminofluorescein diacetate (DAF-2DA), which was recently developed for the detection of intracellular nitric oxide (NO) in living cells, we examined the sensitivity of
Orthogonality of Calcium Concentration and Ability of 4,5-Diaminofluorescein to Detect NO*
DAF-2 can provide reliable information on NO production in biological systems regardless of the dynamic changes of Ca2+concentration, according to a recent report of divalent cation sensitivity and photoactivation of Daf-2.
Nitrosative stress by cyclosporin A in the endothelium: studies with the NO-sensitive probe diaminofluorescein-2/diacetate using flow cytometry.
  • J. Navarro-Antolín, S. Lamas
  • Biology
    Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
  • 2001
Data is provided showing that intracellular NO can be detected by flow cytometry with DAF-2/DA and suggesting two potential mechanisms (transcriptional and post-translational) for the intrace cellular accumulation of NO in vascular endothelial cells exposed to the immunosuppressant CSA.
4,5-diaminofluoroscein imaging of nitric oxide synthesis in crayfish terminal ganglia.
It is found that preincubation with the nitric oxide synthase (NOS) inhibitor L-NAME prevented much of the initial development of DAF-2 fluorescence, whereas the inactive isomer D-NAME had no effect.
Distinction between Nitrosating Mechanisms within Human Cells and Aqueous Solution*
The results suggest that the functional effects of nitrosation may be organized to occur within discrete domains or compartments in biological systems when scavengers are depleted and this architecture becomes compromised.
Chemical nature of nitric oxide storage forms in rat vascular tissue
Endothelial NO production results in local formation of adducts that may act as storage forms of NO. Because little is known about their chemical nature, concentrations, and possible role in vascular