Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin-biotin attachment: a comparative study.

@article{Wacker2004PerformanceOA,
  title={Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin-biotin attachment: a comparative study.},
  author={Ron Wacker and Hendrik V Schr{\"o}der and Christof M. Niemeyer},
  journal={Analytical biochemistry},
  year={2004},
  volume={330 2},
  pages={
          281-7
        }
}
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158 Citations
Highly Influential Citations
2
Background Citations
24
Methods Citations
11
Results Citations
2

158 Citations

DNA-Directed Antibody Immobilization for Robust Protein Microarrays: Application to Single Particle Detection 'DNA-Directed Antibody Immobilization.
TLDR
The design and the use of DNA-directed immobilized (DDI) antibodies in fabrication of robust protein microarrays for capturing and detecting nanoparticles such as intact viruses are presented.
Dual improvement of DNA-directed antibody immobilization utilizing magnetic fishing and a polyamine coated surface
TLDR
An attractive approach with DNA-directed antibody immobilization using a poly-L-lysine coated surface with highly pre-adsorbed ssDNA, along with magnetic nanoparticles to increase the surface concentration of the biomolecules, is promising for the utilization in, and the improvement of, surface-based biosensors.
Orientation and characterization of immobilized antibodies for improved immunoassays (Review).
TLDR
This review describes the most recent methods for oriented antibody immobilization and the characterization techniques employed for investigation of the antibody state.
Spatially addressable protein array: ssDNA‐directed assembly for antibody microarray
TLDR
The design and fabrication of a robust platform, spatially addressable protein array (SAPA) is described by exploring the specificity of ssDNA hybridization for self‐assembly of semi‐synthetic ssDNA‐antibody conjugates which capture antigens from complex biological samples.
DNA-encoding to improve performance and allow parallel evaluation of the binding characteristics of multiple antibodies in a surface-bound immunoassay format.
TLDR
It is shown that DNA-encoding offers advantages in terms of antigen binding capacity, compared to covalently tethered antibodies; and this linkage modality facilitates the rapid self-assembly of multiplexed arrays on account of complementarity between the DNA sequences on the antibodies and sensor array, respectively.
Addressable microfluidic polymer chip for DNA-directed immobilization of oligonucleotide-tagged compounds.
TLDR
DNA-directed immobilization (DDI) of DNA-modified gold nanoparticles (AuNPs) and DNA-conjugates of the enzymes glucose oxidase and horseradish peroxidase are carried out to demonstrate site selectivity and reversibility of the surface-modification process.
DNA-Directed Antibody Immobilization for Enhanced Detection of Single Viral Pathogens.
TLDR
Using DNA conjugated anti-EBOV GP (Ebola virus glycoprotein) improves the sensitivity of EBOV-GP carrying VSV detection compared to directly immobilized antibodies, and utilizing a DNA surface for conversion to an antibody array offers an easier manufacturing process by replacing the antibody printing step with DNA printing.
DNA-directed assembly of protein microarrays.
TLDR
The challenges faced during the design, fabrication, and utilization of protein microarrays are explored and how DNA-directed assembly (DDA) strategies are accelerating the development of platforms available for protein microarray applications are highlighted.
Analytical Devices Based on Direct Synthesis of DNA on Paper.
TLDR
This method starts with the synthesis of DNA oligonucleotides covalently linked to paper and proceeds to assemble microzones of DNA-conjugated paper into arrays capable of simultaneously capturing DNA, DNA- Conjugated protein antigens, and DNA-Conjugated antibodies.
DNA nanostructure-based universal microarray platform for high-efficiency multiplex bioanalysis in biofluids.
TLDR
This study has developed a novel approach for the fabrication of DNA tetrahedron-based microarrays and a universal DNA tetraceuticals platform for the detection of different types of bioactive molecules and demonstrated that the microarray platform worked well with clinical serum samples and showed good relativity with conventional chemical luminescent immunoassay.
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References

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DNA-Directed immobilization: efficient, reversible, and site-selective surface binding of proteins by means of covalent DNA-streptavidin conjugates.
TLDR
Quantitative measurements on microplates indicate that DDI proceeds with a higher immobilization efficiency than conventional immobilization techniques, such as the binding of the biotinylated proteins to streptavidin-coated surfaces or direct physisorption, and simultaneous immobilization of different compounds using microstructured oligonucleotide arrays as immobilization matrices demonstrate that D DI proceeds with site selectivity due to the unique specificity of Watson-Crick base pairing.
Toward optimized antibody microarrays: a comparison of current microarray support materials.
Oligonucleotide-directed self-assembly of proteins: semisynthetic DNA--streptavidin hybrid molecules as connectors for the generation of macroscopic arrays and the construction of supramolecular bioconjugates.
TLDR
The use of nucleic acid sequences as a template for the formation of an array of proteins is further demonstrated on two size scales, and the generation of supramolecular bioconjugates was shown by quantitative measurements and gel-retardation assays.
DDI‐μFIA—A Readily Configurable Microarray‐Fluorescence Immunoassay Based on DNA‐Directed Immobilization of Proteins
TLDR
A chip‐based immunoassay for multiplex antigen detection, based on the self‐assembly of semi‐synthetic DNA–protein conjugates to generate an easily configurable protein microarray, and a method for the internal standardization of protein micro array analyses, to compensate for interexperimental variations usually occurring in microarray analyses.
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TLDR
A novel method for the surface immobilization of DNA using pre-fabricated polyamidoamine (PAMAM) starburst dendrimers as mediator moieties with a very high immobilization efficiency for amino-modified DNA-oligomers and a remarkable high stability during repeated regeneration and re-using cycles is reported.
Dendrimer‐Activated Solid Supports for Nucleic Acid and Protein Microarrays
TLDR
The results indicate that the novel dendrimer‐activated surfaces display a surface coverage with capture oligomers about twofold greater than that with conventional microarrays containing linear chemical linkers.
Antibody arrays for high-throughput screening of antibody–antigen interactions
TLDR
The results indicate that antibody arrays can be used to identify differentially expressed proteins.
Next generation of protein microarray support materials: evaluation for protein and antibody microarray applications.
How to make a DNA chip.
TLDR
With good experience in this initial use, many further applications of microarrays are being developed, including genotyping in research and genetic diagnosis in medicine, and technologies from physical sciences and engineering are now being applied to DNA chips.
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