Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin-biotin attachment: a comparative study.

@article{Wacker2004PerformanceOA,
  title={Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin-biotin attachment: a comparative study.},
  author={Ron Wacker and Hendrik V Schr{\"o}der and Christof M. Niemeyer},
  journal={Analytical biochemistry},
  year={2004},
  volume={330 2},
  pages={
          281-7
        }
}
DNA-Directed Antibody Immobilization for Robust Protein Microarrays: Application to Single Particle Detection 'DNA-Directed Antibody Immobilization.
TLDR
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TLDR
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TLDR
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DNA-directed assembly of protein microarrays.
TLDR
The challenges faced during the design, fabrication, and utilization of protein microarrays are explored and how DNA-directed assembly (DDA) strategies are accelerating the development of platforms available for protein microarray applications are highlighted.
Analytical Devices Based on Direct Synthesis of DNA on Paper.
TLDR
This method starts with the synthesis of DNA oligonucleotides covalently linked to paper and proceeds to assemble microzones of DNA-conjugated paper into arrays capable of simultaneously capturing DNA, DNA- Conjugated protein antigens, and DNA-Conjugated antibodies.
DNA nanostructure-based universal microarray platform for high-efficiency multiplex bioanalysis in biofluids.
TLDR
This study has developed a novel approach for the fabrication of DNA tetrahedron-based microarrays and a universal DNA tetraceuticals platform for the detection of different types of bioactive molecules and demonstrated that the microarray platform worked well with clinical serum samples and showed good relativity with conventional chemical luminescent immunoassay.
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TLDR
Quantitative measurements on microplates indicate that DDI proceeds with a higher immobilization efficiency than conventional immobilization techniques, such as the binding of the biotinylated proteins to streptavidin-coated surfaces or direct physisorption, and simultaneous immobilization of different compounds using microstructured oligonucleotide arrays as immobilization matrices demonstrate that D DI proceeds with site selectivity due to the unique specificity of Watson-Crick base pairing.
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TLDR
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