Performance of a real-time PCR assay in routine bovine mastitis diagnostics compared with in-depth conventional culture.

@article{Hiiti2015PerformanceOA,
  title={Performance of a real-time PCR assay in routine bovine mastitis diagnostics compared with in-depth conventional culture.},
  author={Heidi Hiiti{\"o} and Rauna Riva and Tiina Autio and Tarja Pohjanvirta and Jani Holopainen and Satu Py{\"o}r{\"a}l{\"a} and Sinikka Pelkonen},
  journal={The Journal of dairy research},
  year={2015},
  volume={82 2},
  pages={200-8}
}
Reliable identification of the aetiological agent is crucial in mastitis diagnostics. Real-time PCR is a fast, automated tool for detecting the most common udder pathogens directly from milk. In this study aseptically taken quarter milk samples were analysed with a real-time PCR assay (Thermo Scientific PathoProof Mastitis Complete-12 Kit, Thermo Fisher Scientific Ltd.) and by semi-quantitative, in-depth bacteriological culture (BC). The aim of the study was to evaluate the diagnostic… CONTINUE READING