Performance of a kinetic model for intracellular ice formation based on the extent of supercooling.

@article{Pitt1992PerformanceOA,
  title={Performance of a kinetic model for intracellular ice formation based on the extent of supercooling.},
  author={Robert E. Pitt and M. Chandrasekaran and John S Parks},
  journal={Cryobiology},
  year={1992},
  volume={29 3},
  pages={359-73}
}
Cryomicroscopy was used to study the incidence of intracellular ice formation (IIF) in protoplasts isolated from rye (Secale cereale) leaves during subfreezing isothermal periods and in in vitro mature bovine oocytes during cooling at constant rates. IIF in protoplasts occurred at random times during isothermal periods, and the kinetics of IIF were faster as isothermal temperature decreased. Mean IIF times decreased from approximately 1700 s at -4.0 degrees C to less than 1 s at -18.5 degrees C… CONTINUE READING