Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Subtypes, Group O, and HIV-2

@article{Abravaya2000PerformanceOA,
  title={Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Subtypes, Group O, and HIV-2},
  author={Klara Abravaya and Claudia A. Esping and Robert J. Hoenle and Jacek J. Gorzowski and Robert Perry and Paul E. Kroeger and John Robinson and Richard Flanders},
  journal={Journal of Clinical Microbiology},
  year={2000},
  volume={38},
  pages={716 - 723}
}
ABSTRACT Early detection of human immunodeficiency virus (HIV) in blood and blood products can be achieved by a sensitive nucleic acid amplification-based assay. We report on the performance of a PCR-based qualitative assay that detects both HIV type 1 (HIV-1) and HIV-2 with a sensitivity of 20 to 50 copies/ml. The assay has a specificity of 99.6% and an inhibition rate of 1.7%. One milliliter of sample is processed with a manifold system and Qiagen columns, and one-third of the extracted… Expand
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