Performance of 133 compounds in the lambda prophage induction endpoint of the Microscreen assay and a comparison with S. typhimurium mutagenicity and rodent carcinogenicity assays.

@article{Rossman1991PerformanceO1,
  title={Performance of 133 compounds in the lambda prophage induction endpoint of the Microscreen assay and a comparison with S. typhimurium mutagenicity and rodent carcinogenicity assays.},
  author={T. Rossman and Mar{\'i}a Molina and Lisa Meyer and Pamela Boone and Catherine B. Klein and Zhigang Wang and Feng Li and Wei Ju Lin and Patrick L Kinney},
  journal={Mutation research},
  year={1991},
  volume={260 4},
  pages={349-67}
}
The Microscreen assay was developed as a means of testing very small samples, as in complex mixture fractionation. It is a multi-endpoint assay which utilizes E. coli WP2s(lambda). Exposure takes place to serial dilutions of the test compound in microtitre wells (250 microliters) followed by sampling from wells in which growth has occurred ('non-toxic wells'). Although a number of different endpoints can be measured, only the prophage induction endpoint (the first one developed) has been… CONTINUE READING