Performance evaluation of five commercial real-time PCR reagent systems using TaqMan assays for B. anthracis detection.

@article{Sohni2008PerformanceEO,
  title={Performance evaluation of five commercial real-time PCR reagent systems using TaqMan assays for B. anthracis detection.},
  author={Youvraj Sohni and Sagarika Kanjilal and Vivek Kapur},
  journal={Clinical biochemistry},
  year={2008},
  volume={41 7-8},
  pages={640-4}
}
BACKGROUND Real-time PCR assay sensitivity is affected by the choice and concentrations of reaction mix constituents among other factors such as primers, probes, and analytical assay platforms. Commercially available reagent mixes facilitate PCR assay set-up with fewer steps and timeliness. However, determination of analytical assay framework is important for ready-to-use real-time PCR reagent systems for rapid, quantitative and accurate detection of bioterror pathogens such as Bacillus… CONTINUE READING

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