• Corpus ID: 8612272

Performance comparison of a fliC(h7) real-time PCR assay with an H7 latex agglutination test for confirmation of the H type of Escherichia coli O157:H7.

@article{Narang2009PerformanceCO,
  title={Performance comparison of a fliC(h7) real-time PCR assay with an H7 latex agglutination test for confirmation of the H type of Escherichia coli O157:H7.},
  author={Neelam Narang and Pina Fratamico and Glenn E. Tillman and Kitty Pupedis and William C. Cray},
  journal={Journal of food protection},
  year={2009},
  volume={72 10},
  pages={
          2195-7
        }
}
Escherichia coli O157:H7 is a foodborne pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. Positive identification of E. coli O157:H7 is made using biochemical tests and specific antisera or latex agglutination reagents for the O157 and H7 antigens. However, under certain conditions, some E. coli O157:H7 isolates can appear to be nonreactive with H7 antisera and may require multiple passages on motility medium to restore H7 antigenicity. In this study, we compared the… 

Figures and Tables from this paper

Improved PCR assay for the specific detection and quantitation of Escherichia coli serotype O157 in water
TLDR
This study showed that the newly developed quantitative serotype-specific PCR method is a highly specific and efficient tool for the surveillance and rapid detection of high-risk E. coli serotype O157 in environmental water samples.
Development of a sensitive detection method for stressed E. coli O157:H7 in source and finished drinking water by culture-qPCR.
TLDR
The results suggest that the culture-PCR procedure can be used for rapid detection of E. coli O157:H7 in drinking water.
Prevalence of Escherichia coli O157 in red meat and meat products determined by VIDAS ECPT and LightCycler PCR
TLDR
The 5.55% prevalence of E. coli O157 in red meats poses a significant risk for consumers and indicates insufficient hygiene management both at the farm and during the slaughtering and meat handling processes in Turkey.
Development and Evaluation of a Simple Latex Agglutination Test for the Detection of Pig Antibodies Against Human T-cell
TLDR
A simple and rapid latex agglutination test was developed to detect pig antibodies against human T-cell instead of the time-consuming erythrocyte rosette inhibition test (ERIT).
Rapid Waterborne Pathogen Detection with Mobile Electronics
TLDR
An immunoagglutination-based protocol together with the microfluidic device to quantify pathogen levels directly from water samples to enable field detection for waterborne pathogens is proposed.

References

SHOWING 1-10 OF 12 REFERENCES
Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NM
TLDR
A PCR-RFLP test developed in conjunction with O serogroup determination will be useful in identifying E. coli O157:H7 and related strains that do not express immunoreactive H antigen and could be expanded to include other clinically importantE.
Evaluation of commercial latex reagents for identification of O157 and H7 antigens of Escherichia coli
TLDR
These findings demonstrate that the commercial latex reagents are good alternatives to standard serologic methods for identifying the O157 and H7 antigens of E. coli.
Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis
TLDR
SMAC medium stool culture is a simple, inexpensive, rapid, and reliable means of detecting E. coli O157:H7, and it is recommended routine use of SMAC medium especially for culturing bloody stools.
Detection of Escherichia coli O157:H7 in Food Using Real-Time Multiplex PCR Assays Targeting the stx1, stx2, wzyO157, and the fliCh7 or eae Genes
TLDR
The real-time multiplex PCR assays targeting the stx1, stx2, eae, and wzyO157 or the fliCh7 genes are sensitive and specific and can be used for the detection of E. coli O157:H7 in food, except that the fl iCh7 gene may not be a suitable target for the Detection of E.'s coli O 157: H7 in ground beef.
Latex agglutination test for detection of Escherichia coli serotype O157
TLDR
It was found that sorbitol-MacConkey agar cultures were not as useful for food samples as they were for fecal specimens in screening for E. coli O157:H7, but the use of the latex screen was particularly efficient in this setting.
Comparison of methods for detection and isolation of cold- and freeze-stressed Escherichia coli O157:H7 in raw ground beef.
TLDR
REB had better overall performance for enrichment of cold- and freeze-stressed E. coli O157:H7 present in ground beef than did the other media examined, and significant main effects of treatment, type of medium, enrichment time, inoculum concentration, and detection method were indicated.
Characterization of nonmotile variants of Escherichia coli O157 and other serotypes by using an antiflagellin monoclonal antibody
An antiflagellin monoclonal antibody (15D8) was used to detect the presence of flagella in nonmotile variants of several pathogenic Escherichia coli serotypes. Of the 48 isolates examined, 15 reacted
Infection by verocytotoxin-producing Escherichia coli.
  • M. Karmali
  • Biology, Medicine
    Clinical Microbiology Reviews
  • 1989
TLDR
The best strategies for diagnosing human VTEC infection including testing for the presence of free VT in fecal filtrates and examining fecal cultures for VTEC by means of deoxyribonucleic acid probes that specify genes encoding VT1 and VT2 are currently confined to specialized laboratories and await commercial development for wider use.
of Agriculture
  • 1998. Detection, isolation, and identification of Escherichia coli O157 and O157:NM (nonmotile) from meat products, chap. 5.04, p. 1–15. Microbiology laboratory guidebook, 3rd ed. U.S. Department of Agriculture, Food Safety and Inspection Service, Washington, DC. Available at: http://www.fsis. usda.
  • 2008
...
1
2
...