Performance characteristics of cholesterol oxidase for kinetic determination of total cholesterol.

  title={Performance characteristics of cholesterol oxidase for kinetic determination of total cholesterol.},
  author={Pornpen Srisawasdi and Patcharee Jearanaikoon and Martin H. Kroll and Porntip H. Lolekha},
  journal={Journal of clinical laboratory analysis},
  volume={19 6},
The enzymatic method for cholesterol determination can use either an endpoint or a kinetic method. Not much is known concerning the properties (K(m) and V(max)) of the commercial enzyme for the kinetic method. We measured the K(m) and V(max) of Brevibacterium, Streptomyces, Pseudomonas fluorescens, and Cellulomonas cholesterol oxidase. Brevibacterium gave the highest K(m) value (230.3 x 10(-4) M), followed by Streptomyces (2.17 x 10(-4) M), Cellulomonas (0.84 x 10(-4) M), and Pseudomonas (0.61… CONTINUE READING
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