Perforated-patch recording with gramicidin avoids artifactual changes in intracellular chloride concentration.

@article{Kyrozis1995PerforatedpatchRW,
  title={Perforated-patch recording with gramicidin avoids artifactual changes in intracellular chloride concentration.},
  author={Andreas Kyrozis and David B. Reichling},
  journal={Journal of neuroscience methods},
  year={1995},
  volume={57 1},
  pages={27-35}
}
The antibiotic gramicidin, when incorporated into lipid membranes, forms pores that are exclusively permeable to monovalent cations and small unchanged molecules. We report the use of gramicidin for perforated patch-clamp recordings in the whole-cell mode. Recordings were performed in cultured rat spinal cord dorsal horn neurons. Cells had stable resting potentials and series resistances for times routinely exceeding 60 min. To test if intracellular chloride concentration ([Cl]i) remains stable… CONTINUE READING
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