Perchlorate and nitrate reductase activity in the perchlorate-respiring bacterium perclace.

@article{Giblin2001PerchlorateAN,
  title={Perchlorate and nitrate reductase activity in the perchlorate-respiring bacterium perclace.},
  author={Tara Giblin and William T. Frankenberger},
  journal={Microbiological research},
  year={2001},
  volume={156 4},
  pages={311-5}
}
The perchlorate (ClO4(-))-respiring organism, strain perclace, can grow using nitrate (NO3(-)) as a terminal electron acceptor. In resting cell suspensions, NO(-) grown cells reduced ClO4(-), and ClO4(-) grown cells reduced NO3(-). Activity assays showed that nitrate reductase (NR) activity was 1.31 micromol min(-1) (mg protein)-1 in (ClO4)- grown cells, and perchlorate reductase (PR) activity was 4.24 micromol min(-1) (mg protein)(-1) in NO3(-) grown cells. PR activity was detected within the… CONTINUE READING