Percentage of cells in the S phase of the cell cycle in human lymphoma determined by flow cytometry.

@article{Braylan1980PercentageOC,
  title={Percentage of cells in the S phase of the cell cycle in human lymphoma determined by flow cytometry.},
  author={Raul C. Braylan and Lawrence W. Diamond and M. Luann Powell and Barbara Harty-Golder},
  journal={Cytometry},
  year={1980},
  volume={1 3},
  pages={
          171-4
        }
}
The percentage of cells with S phase DNA content (S cells) was determined by flow cytometry in cell suspensions obtained from 27 patients with non-Hodgkin's lymphomas. DNA was measured in ethanol-fixed cells stained with propidium iodide after RNase treatment. Cells from 17 samples were also exposed to [3H]-thymidine and labeling indices were determined. An excellent linear correlation was observed between the percentage of S cells calculated by flow cytometry and the labeling index (r = 0.95… CONTINUE READING
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