Peptidomics of an in vitro digested α-Gal carrying protein revealed IgE-reactive peptides

@article{Apostolovic2017PeptidomicsOA,
  title={Peptidomics of an in vitro digested α-Gal carrying protein revealed IgE-reactive peptides},
  author={D Apostolovic and Milena Krsti{\'c} and Jelena Mihailovic and Maria Starkhammar and T Cirkovic Velickovic and C Hamsten and Marianne van Hage},
  journal={Scientific Reports},
  year={2017},
  volume={7}
}
  • D Apostolovic, Milena Krstić, +4 authors Marianne van Hage
  • Published 2017
  • Chemistry, Medicine
  • Scientific Reports
  • The mammalian carbohydrate galactose-α1,3-galactose (α-Gal) causes a novel form of food allergy, red meat allergy, where patients experience severe allergic reactions several hours after red meat consumption. Here we explored gastric digestion of α-Gal glycoproteins using an in vitro model. Bovine thyroglobulin (BTG), a typical α-Gal carrying glycoprotein, was digested with pepsin. The resulting peptides were characterised by SDS PAGE, immunoblot and ImmunoCAP using sera from 20 red meat… CONTINUE READING

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