Peptidomics-based discovery of novel neuropeptides.

  title={Peptidomics-based discovery of novel neuropeptides.},
  author={Marcus Svensson and Karl Sk{\"o}ld and Per Svenningsson and Per E. Andr{\'e}n},
  journal={Journal of proteome research},
  volume={2 2},
Modern proteomic methodologies have significantly improved the possibilities of large-scale identification of proteins. However, these methodologies are limited by their inability to reliably detect endogenously expressed peptides. We describe a novel approach of combining sample preparation, comprising focused microwave irradiation and mass spectrometric peptide profiling that has enabled us to simultaneously detect more than 550 endogenous neuropeptides in 1 mg of hypothalamic extracts… CONTINUE READING