Peptide thioester preparation by Fmoc solid phase peptide synthesis for use in native chemical ligation.

@article{Clippingdale2000PeptideTP,
  title={Peptide thioester preparation by Fmoc solid phase peptide synthesis for use in native chemical ligation.},
  author={Andrew B. Clippingdale and Colin James Barrow and John D Wade},
  journal={Journal of peptide science : an official publication of the European Peptide Society},
  year={2000},
  volume={6 5},
  pages={225-34}
}
Established methodology for the preparation of peptide thioesters requires the use of t-butoxycarbonyl chemistry owing to the lability of thioester linkers to the nucleophilic reagents used in Fmoc solid phase peptide synthesis. Both the greater ease of use and the broad applicability of the method has led to the development of an Fmoc-based methodology for direct peptide thioester synthesis. It was found that successful preparation of a peptide thioester could be achieved when the non… CONTINUE READING
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