Peptide release promoted by methylated RF2 and ArfA in nonstop translation is achieved by an induced-fit mechanism.

@article{Zeng2016PeptideRP,
  title={Peptide release promoted by methylated RF2 and ArfA in nonstop translation is achieved by an induced-fit mechanism.},
  author={Fuxing Zeng and Hong Jin},
  journal={RNA},
  year={2016},
  volume={22 1},
  pages={49-60}
}
Here we report that the specificity of peptide release in the ribosome on a nonstop mRNA by ArfA and RF2 is achieved by an induced-fit mechanism. Using RF2 that is methylated on the glutamine of its GGQ motif (RF2(m)), we show that methylation substantially increases the rate of ArfA/RF2-catalyzed peptide release on a nonstop mRNA that does not occupy the ribosomal A site, but has only a modest effect on k(cat) by the same proteins on longer nonstop mRNAs occupying the A site of the mRNA… CONTINUE READING
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