Peptide-mediated transcytosis of phage display vectors in MDCK cells.

@article{Ivanenkov2000PeptidemediatedTO,
  title={Peptide-mediated transcytosis of phage display vectors in MDCK cells.},
  author={Vasily V Ivanenkov and Anil G. Menon},
  journal={Biochemical and biophysical research communications},
  year={2000},
  volume={276 1},
  pages={251-7}
}
Delivery of therapeutic macromolecules and gene vectors to certain tissues is hampered by endothelial or epithelial barriers. We show here that the transport of phage particles across epithelial cells can be facilitated by peptide ligands selected from a phage library of random peptides. Using MDCK cells, we identified a polycationic peptide sequence, RYRGDLGRR, containing a putative integrin-binding (RGD) motif that enhanced basal-to-apical transcytosis of peptide-bearing phage 1000- to 10,000… CONTINUE READING

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