Peptide linkage mapping of the Agrobacterium tumefaciens vir-encoded type IV secretion system reveals protein subassemblies.

@article{Ward2002PeptideLM,
  title={Peptide linkage mapping of the Agrobacterium tumefaciens vir-encoded type IV secretion system reveals protein subassemblies.},
  author={Doyle V. Ward and Olga Draper and John R. Zupan and Patricia C. Zambryski},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 17},
  pages={11493-500}
}
Numerous bacterial pathogens use type IV secretion systems (T4SS) to deliver virulence factors directly to the cytoplasm of plant, animal, and human host cells. Here, evidence for interactions among components of the Agrobacterium tumefaciens vir-encoded T4SS is presented. The results derive from a high-resolution yeast two-hybrid assay, in which a library of small peptide domains of T4SS components was screened for interactions. The use of small peptides overcomes problems associated with… CONTINUE READING
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