Peptide hydrolases of the human small intestinal mucosa: distribution of activities between brush border membranes and cytosol.

@article{Sterchi1980PeptideHO,
  title={Peptide hydrolases of the human small intestinal mucosa: distribution of activities between brush border membranes and cytosol.},
  author={Erwin E. Sterchi and Julie Woodley},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1980},
  volume={102 1},
  pages={49-56}
}
Brush border membranes from frozen human small intestine have been purified using a method which did not involve the use of EDTA-containing buffers or the disruption of brush border fragments with high concentrations of Tris. On average a 24-fold increase in specific activity of alpha-glucosidase (brush border marker) was obtained in the final preparation which contained insignificant traces of enzyme marker activities from cytosol and lysosomes. The homogenates of human small intestinal mucosa… CONTINUE READING