Peptide hydrolases of the human small intestinal mucosa: identification of six distinct enzymes in the brush border membrane.

@article{Sterchi1980PeptideHO,
  title={Peptide hydrolases of the human small intestinal mucosa: identification of six distinct enzymes in the brush border membrane.},
  author={Erwin E. Sterchi and Julie Woodley},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1980},
  volume={102 1},
  pages={57-65}
}
For this investigation highly purified brush border membranes from human small intestine, prepared according to the method described in the preceding paper [1], have been used. Solubilisation of brush border membrane proteins by sodium dodecyl sulphate, Triton X-100 and papain followed by electrophoresis in polyacrylamide gels revealed six distinct peptide hydrolases. These included the enzymes aminopeptidase A (EC 3.4.11.7), dipeptidylpeptidase IV (EC 3.4.14.-), gamma-glutamultranspeptidase… CONTINUE READING