Peptide bond formation by in vitro selected ribozymes

@article{Zhang1997PeptideBF,
  title={Peptide bond formation by in vitro selected ribozymes},
  author={Biliang Zhang and Thomas R. Cech},
  journal={Nature},
  year={1997},
  volume={390},
  pages={96-100}
}
An attractive solution to the problem of the origin of protein synthesis in an evolving ‘RNA world’ involves catalysis by nucleic acid without assistance from proteins,. Indeed, even the modern ribosome has been considered to be fundamentally an RNA machine, and the large ribosomal subunit can carry out peptidyl transfer in the absence of most of its protein subunits. Successive cycles of in vitro selection and amplification have been used to find RNAs that perform many biochemical reactions… Expand
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  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2001
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References

SHOWING 1-10 OF 56 REFERENCES
Ribozyme-catalysed amino-acid transfer reactions
TLDR
In vitro selection and evolution is used to isolate ribozymes with acyl transferase activity from a pool of random RNA sequences and one of them transfers an amino acid to itself in a reaction that is analogous to peptidyl transfer on the ribosome. Expand
In vitro evolution of a self-alkylatlng ribozyme
TLDR
RNA enzymes are postulated to have catalysed all chemical reactions in the earliest living cells, and selection for self-biotinylation yields a transfer RNA-like ribozyme that efficiently catalyses carbon–nitrogen bond formation. Expand
Aminoacyl esterase activity of the Tetrahymena ribozyme.
TLDR
The ability of RNA to catalyze reactions with aminoacyl esters expands the catalytic versatility of RNA and suggests that the first aminoacyL tRNA synthetase could have been an RNA molecule. Expand
Cleavage of an amide bond by a ribozyme
A variant form of a group I ribozyme, optimized by in vitro evolution for its ability to catalyze magnesium-dependent phosphoester transfer reactions involving DNA substrates, also catalyzes theExpand
Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA
TLDR
The selected molecule represents the discovery of the first RNA enzyme known to cleave single-stranded DNA specifically and allows evolution experiments to be carried out in response to artificially imposed selection constraints. Expand
Isolation of new ribozymes from a large pool of random sequences [see comment].
TLDR
An iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs (ribozymes) from a large pool of random-sequence RNA molecules, leading to improvement of the average ligation activity and the emergence of ribozymes with reaction rates 7 million times faster than the uncatalyzed reaction rate. Expand
In vitro evolution of new ribozymes with polynucleotide kinase activity
TLDR
An engineered version of one class of kinases is able to catalyse the transfer of thiophosphate from ATP-γS to the 5′-hydroxyl of an exogenous oligoribonucleotide substrate with multiple turnover, thus acting as a true enzyme. Expand
In vitro selection of a novel catalytic RNA: characterization of a sulfur alkylation reaction and interaction with a small peptide.
TLDR
An in vitro RNA selection for catalytic activity was used to co-select for binding activity to a small peptide, showing that the intrinsic reactivity of the 5' phosphorothioate is not increased in the selected RNA. Expand
RNA-catalysed RNA polymerization using nucleoside triphosphates
TLDR
An RNA is described that synthesizes RNA using the same reaction as that employed by protein enzymes that catalyse RNA polymerization, and shows marked template fidelity. Expand
Aminoacyl-RNA synthesis catalyzed by an RNA
TLDR
An RNA has been selected that rapidly aminoacylates its 2'(3') terminus when provided with phenylalanyl-adenosine monophosphate and this results confirm a necessary prediction of the RNA world hypothesis and represent efficient RNA reaction at a carbonyl carbon. Expand
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5
...