The ErbB2 receptor tyrosine kinase is overexpressed in approximately 30% of breast tumor cases and its overexpression correlates with an unfavorable prognosis. A major contributor for this course of the disease is the insensitivity of these tumors toward chemotherapy. Monoclonal antibodies, inhibiting the ligand-induced activation of the receptor and… (More)
FIGURE 7. Efficient gene transfer and aptamer expression in 293T and MCF7 cells on lentiviral gene transduction. A. The aptamer fusion proteins were integrated into lentiviral expression vectors, pSiEW, with 5¶and 3¶ long terminal repeat (LTR), spleen focus-forming virus (SFFV ) promoter, Flag-tagged aptamer-thioredoxin expression cassette, internal ribosome entry site (IRES ), EGFP coding sequence, and woodchuck posttranscriptional regulatory element (WPRE ). B. Lentiviral particles encoding thioredoxin, Ag-11, and AII-7 were obtained from transfected helper cells and used for the infection of 293T, MCF7, MCF7-neo, MCF7-her2, and SKBR3 cells. Infection efficiency was measured by EGFP expression of infected cells in a fluorescence activated cell sorter. C. 293T cells infected with the lentiviral expression vectors for thioredoxin, Ag-11, and AII-7 were lysed and the virally encoded proteins were visualized after gel electrophoresis and Western blot analysis.