Pepsin and Carbonic Anhydrase Isoenzyme III as Diagnostic Markers for Laryngopharyngeal Reflux Disease

  title={Pepsin and Carbonic Anhydrase Isoenzyme III as Diagnostic Markers for Laryngopharyngeal Reflux Disease},
  author={Nikki Johnston and John Knight and Peter William Dettmar and Mark O. Lively and James A. Koufman},
  journal={The Laryngoscope},
Objectives/Hypothesis: The objective was to investigate the potential use of pepsin and carbonic anhydrase isoenzyme III (CA‐III) as diagnostic markers for laryngopharyngeal reflux disease. 
Reflux changes in adenoidal hyperplasia: a controlled prospective study to investigate its aetiology
  • P. K. Harris, D. Hussey, +6 authors A. Carney
  • Medicine
    Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery
  • 2009
Objectives:  To compare pepsin, carbonic anhydrase III (CAIII), cyclooxygenase‐2 (COX‐2) and mucin 5AC (MUC5AC) expression in children with adenoid hypertrophy and normal controls.
Bile acids in laryngopharyngeal refluxate: Will they enhance or attenuate the action of pepsin?
To assess if, as previously reported in the literature, bile acids inhibit pepin activity, resulting in pepsin having a less important role in laryngopharyngeal damage in reflux disease, biliary acid inhibition is reported to have a role in this disease.
Activity/Stability of Human Pepsin: Implications for Reflux Attributed Laryngeal Disease
Whether pepsin has to be enzymatically active to deplete laryngeal protective proteins, carbonic anhydrase isoenzyme III (CAIII), and squamous epithelial stress protein Sep70 is determined.
The presence of pepsin in the lung and its relationship to pathologic gastro‐esophageal reflux
If there is a higher reflux burden as measured by pH‐MII in patients that are pepsin positive in the lung, and the sensitivity of pepsin in predicting pathologic reflux by pH, MII, and EGD is studied.
Immunohistochemical detection of pepsin in laryngeal mucosa for diagnosing laryngopharyngeal reflux
To investigate whether the pepsin immunohistochemical (IHC) staining of the laryngeal mucosa epithelia is an available test for diagnosing laryngopharyngeal reflux (LPR) in clinic.
Receptor Mediated Uptake of Pepsin: Significance in Otolaryngology
Abstract Reflux of gastric contents into the laryngopharynx contributes to voice disorders, otolaryngological inflammatory disorders, and perhaps even neoplastic diseases of the laryngopharynx. Tre...
Saliva Pepsin Concentration of Laryngopharyngeal Reflux Patients Is Influenced by Meals Consumed Before the Samples
The objective was to assess the impact of diet on the saliva pepsin concentration of patients with laryngopharyngeal reflux and to establish a smoking cessation strategy for patients with LPR.
The Role of Pepsin in Laryngopharyngeal Reflux
This review focuses on the current studies about pepsin as a specific marker for LPR and putative relationship between pepingin and laryngeal cancer.
Optimal timing of saliva collection to detect pepsin in patients with laryngopharyngeal reflux
To determine salivary pepsin levels as a function of collection time, a single sample of saliva from eight adults was collected over a 12-week period to establish a baseline level.
Pepsin as a Marker of Extraesophageal Reflux
Pepsin assay is a useful tool for correlation of reflux with airway disease and is a reliable diagnostic marker of EER.


The Otolaryngologic Manifestations of Gastroesophageal Reflux Disease (GERD): A Clinical Investigation of 225 Patients Using Ambulatory 24‐Hour pH Monitoring and an Experimental Investigation of the Role of Acid and Pepsin in the Development of Laryngeal Injury
A new diagnostic technique is applied to a population of otolaryngology patients with GERD to determine the incidence of overt and occult GERD and the potential damaging effects of intermittent GER on the larynx are evaluated.
Cell Biology of Laryngeal Epithelial Defenses in Health and Disease: Preliminary Studies
The laryngeal epithelium expresses some CA isoenzymes and has the potential to protect itself against laryngopharyngeAL reflux and may be more sensitive to injury due to reflux damage than the esophageal mucosa because of different responses of CA isenzymes.
Reflux as an etiological factor of carcinoma of the laryngopharynx
The common presence of GER in nonsmoking, nondrinkers or only light social drinkers with moderate to severe gastroesophageal reflux and the probable role of this chronic irritative disorder as a causative agent in carcinoma of the laryngopharynx is discussed.
pH stability and activity curves of pepsin with special reference to their clinical importance.
The present study was undertaken of the effect ofpH on the stability and activity of pepsin.
Is Chronic Gastroesophageal Reflux a Causative Factor in Glottic Carcinoma?
  • M. Morrison
  • Medicine
    Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery
  • 1988
A small number of cases of glottic carcinoma are presented, all with T1 lesions of the anterior two-thirds of the vocal cord, and it is important for the otolaryngologic community to be aware of the possible clinical relationship.
Cell Biology of Laryngeal Epithelial Defenses in Health and Disease: Further Studies
The laryngeal epithelium lacks defenses comparable to those in esophageal epithellium, and these differences may contribute to the increased susceptibility of laryngesis to reflux-related injury.
Laryngospasm and reflex central apnoea caused by aspiration of refluxed gastric content in adults.
Investigations showed that amyotrophic lateral sclerosis was the cause of the oesophageal motility disorder and an intense antireflux treatment abolished the respiratory attacks in both patients.
Acid neutralizing capacity of human saliva.
Saliva produced at physiologic rates and carried into the esophagus by swallowing is capable of neutralizing small amounts of intraesophageal acid within a few minutes, and the ability of saliva to neutralize acid is due primarily to bicarbonate.
Serosal bicarbonate protects against acid injury to rabbit esophagus.
It is concluded that serosal HCO3- is important in esophageal protection against acid damage by buffering H+ within the intercellular compartment of the extracellular space.
Active pepsin in airway secretions : possible evidence for new supraesophageal pH crite
  • Gastroenterology
  • 2001