Pepper β-Galactosidase 1 (PBG1) Plays a Significant Role in Fruit Ripening in Bell Pepper (Capsicum annuum)

@article{Ogasawara2007Pepper1,
  title={Pepper $\beta$-Galactosidase 1 (PBG1) Plays a Significant Role in Fruit Ripening in Bell Pepper (Capsicum annuum)},
  author={Satoshi Ogasawara and Keietsu Abe and Tasuku Nakajima},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2007},
  volume={71},
  pages={309 - 322}
}
During bell pepper (Capsicum annuum L.) fruit ripening, β-galactosidase activity increased markedly as compared with other glycosidases. We purified 77.5 kDa exo-1,4-β-D-galactanase from red bell pepper fruit classified as β-galactosidase II. A marked decrease in galactose content appeared during fruit ripening, especially in the pectic fraction. The purified enzyme hydrolyzed a considerable amount of galactose residues in this fraction. We isolated bell pepper β-galactosidase (PBG1) cDNA. This… 
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References

SHOWING 1-10 OF 76 REFERENCES
β-Galactosidase and Its Significance in Ripening of “Saijyo” Japanese Persimmon Fruit
The fruit extracts of ripening cv. Japanese Persimmon, “Saijyo”, contained a number of glycosidases and glycanases. Among them, β-galactosidase appeared to be the most significant, and the activity…
Papaya beta-galactosidase/galactanase isoforms in differential cell wall hydrolysis and fruit softening during ripening.
Tomato Exo-(1->4)-[beta]-D-Galactanase (Isolation, Changes during Ripening in Normal and Mutant Tomato Fruit, and Characterization of a Related cDNA Clone)
TLDR
An exo-(1->4)-[beta]-D-galactanase was isolated from ripe tomato fruit using anion-ex exchange, gel filtration, and cation-exchange chromatography, and Northern analysis demonstrated that pTom[beta]gal 1 expression was ripening related in normal fruits, with lower levels apparent in the nonsoftening mutants.
β-Galactosidase from Coffea Arabica and its role in fruit ripening
beta-Galactosidases with a lectin-like domain are expressed in strawberry.
TLDR
Strawberry fruits undergo a marked softening during their ripening, and the process is accompanied by a release of free sugars with galactose among them, and total beta-galactosidase activity was measured in cell wall proteins from strawberry fruits at different developmental stages.
The Role of beta-Galactosidases in the Modification of Cell Wall Components during Muskmelon Fruit Ripening.
TLDR
Molecular sizes of pectic polymers and hemicelluloses extracted from fruit mesocarp cell walls were shifted from larger to smaller polymers during ripening, as determined by gel filtration profiles.
β-Galactosidases in Ripening Tomatoes
TLDR
The ability of β-galactosidase II to degrade the galactan and the increase in its activity during tomato ripening suggest a possible role for this enzyme in tomato softening.
beta-Galactosidase and its significance in ripening mango fruit.
Endo-1,4-[beta]-Glucanase, Xyloglucanase, and Xyloglucan Endo-Transglycosylase Activities Versus Potential Substrates in Ripening Tomatoes
TLDR
Although it disappeared from ripe fruit, XGase may have functioned in promoting wall loosening at earlier stages of fruit development when its activity was highest, and depolymerizing (XGase) capacity differed from [beta]-glucanase and XG transglycosylase activity by being almost entirely buffer insoluble, and by declining precipitously during fruit softening.
A gene coding for tomato fruit beta-galactosidase II is expressed during fruit ripening. Cloning, characterization, and expression pattern.
TLDR
The cloning of a cDNA, pTombetagal 4, corresponding to beta-galactosidase II is reported, and it is shown that its corresponding gene is expressed during fruit ripening and suggested a high probability for secretion based on the presence of a hydrophobic leader sequence, a leader-sequence cleavage site, and three possible N-glycosylation sites.
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