Pentatricopeptide Repeat Proteins in Trypanosoma brucei Function in Mitochondrial Ribosomes

@article{Pusnik2007PentatricopeptideRP,
  title={Pentatricopeptide Repeat Proteins in Trypanosoma brucei Function in Mitochondrial Ribosomes},
  author={M. Pusnik and I. Small and L. Read and T. Fabbro and A. Schneider},
  journal={Molecular and Cellular Biology},
  year={2007},
  volume={27},
  pages={6876 - 6888}
}
ABSTRACT The pentatricopeptide repeat (PPR), a degenerate 35-amino-acid motif, defines a novel eukaryotic protein family. Plants have 400 to 500 distinct PPR proteins, whereas other eukaryotes generally have fewer than 5. The few PPR proteins that have been studied have roles in organellar gene expression, probably via direct interaction with RNA. Here we show that the parasitic protozoan Trypanosoma brucei encodes 28 distinct PPR proteins, an extraordinarily high number for a nonplant organism… Expand
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References

SHOWING 1-10 OF 52 REFERENCES
Identification of pentatricopeptide repeat proteins in Trypanosoma brucei.
TLDR
Preliminary characterization suggests that PPR proteins will play important roles in the complex RNA processing required for mitochondrial gene expression in trypanosomes. Expand
A chloroplast-localized PPR protein required for plastid ribosome accumulation.
TLDR
Results suggest that ppr2 functions in the synthesis or assembly of one or more component of the plastid translation machinery, some of which may be associated with RNA. Expand
The multifarious roles of PPR proteins in plant mitochondrial gene expression
TLDR
Much of the recent interest in the role of PPR proteins in mitochondria has been driven by the discovery that most cytoplasmic male sterility systems comprise fertility restorer genes that are members of this fascinating family. Expand
Chloroplast RNA-binding and pentatricopeptide repeat proteins.
TLDR
This study focuses on two RNA-binding proteins: cpRNP (chloroplast ribonucleoprotein) and PPR (pentatricopeptide repeat) protein, suggested to be major contributors to chloroplast RNA metabolism. Expand
The PPR motif - a TPR-related motif prevalent in plant organellar proteins.
TLDR
This large gene family (almost 200 genes in the 70% of the Arabidopsis genome sequenced so far) is characterized by the presence of tandem arrays of a degenerate 35-amino-acid repeat, and it is proposed to call them PPR (pentatricopeptide) motifs. Expand
Trypanosoma brucei mitochondrial ribonucleoprotein complexes which contain 12S and 9S ribosomal RNAs.
TLDR
Using this system, it is confirmed the existence of a prokaryotic-type translational activity being cycloheximide resistant, chloramphenicol sensitive and streptomycin dependent, analogous to what has been found for other eukarya as the independent protein synthesis activity of the mitochondrial organelle. Expand
Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis
TLDR
A detailed bioinformatic analysis of 441 members of the Arabidopsis PPR family plus genomic and genetic data on the expression, localization, and general function of many family members confirm, but massively extend, the very sparse observations previously obtained from detailed characterization of individual mutants in other organisms. Expand
Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae.
TLDR
The combined mass spectrometry and electron microscopy data indicate that the majority of 45S SSU* particles represents a heterodimeric complex in which the SSU of the Leishmania mitochondrial ribosome is associated with an additional protein mass. Expand
RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA.
TLDR
The nuclear-encoded HCF152 was characterized to be a chloroplast RNA-binding protein that may be involved in the processing or stabilization of the petB transcript by binding to the exon-intron junctions. Expand
Further characterization of the extremely small mitochondrial ribosomal RNAs from trypanosomes: a detailed comparison of the 9S and 12S RNAs from Crithidia fasciculata and Trypanosoma brucei with rRNAs from other organisms.
TLDR
The results provide unequivocal evidence for a ribosomal function of the 9S and 12S RNAs of trypanosomal mitochondria, notwithstanding their extremely small size and their unusual base composition. Expand
...
1
2
3
4
5
...