Penetratin-Mediated Transepithelial Insulin Permeation: Importance of Cationic Residues and pH for Complexation and Permeation

@article{Kristensen2015PenetratinMediatedTI,
  title={Penetratin-Mediated Transepithelial Insulin Permeation: Importance of Cationic Residues and pH for Complexation and Permeation},
  author={Mie Kristensen and Henrik Franzyk and Mia Thorne Klausen and Anne Kristine Servais Iversen and Jesper S\oborg Bahnsen and Rikke Bjerring Skyggebjerg and Vito Foder{\`a} and Hanne M\orck Nielsen},
  journal={The AAPS Journal},
  year={2015},
  volume={17},
  pages={1200-1209}
}
Penetratin is a widely used carrier peptide showing promising potential for mucosal delivery of therapeutic proteins. In the present study, the importance of specific penetratin residues and pH was investigated with respect to complexation with insulin and subsequent transepithelial insulin permeation. Besides penetratin, three analogues were studied. The carrier peptide-insulin complexes were characterized in terms of size and morphology at pH 5, 6.5, and 7.4 by dynamic light scattering (DLS… CONTINUE READING

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