Penaeus monodon nucleopolyhedrovirus detection using an immunochromatographic strip test.

@article{Wangman2012PenaeusMN,
  title={Penaeus monodon nucleopolyhedrovirus detection using an immunochromatographic strip test.},
  author={Pradit Wangman and Siwaporn Longyant and Parin Chaivisuthangkura and Pattarin Sridulyakul and Sombat Rukpratanporn and Paisarn Sithigorngul},
  journal={Journal of virological methods},
  year={2012},
  volume={183 2},
  pages={210-4}
}
An immunochromatographic strip test is described for detection of the polyhedrin protein of Penaeus monodon nucleopolyhedrovirus (PemoNPV). The test employs one monoclonal antibody (MAb MBV5) conjugated to colloidal gold to bind to polyhedrin protein and a 1:1:1 mixture of 3 other MAbs (MBV8, 14 and 21) to capture colloidal-gold MAb-protein complexes at a test (T) line on the nitrocellulose strip. A downstream control (C) line of goat anti-mouse immunoglobulin G (GAM) antibody is used to… CONTINUE READING

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