Peculiarities of Herpes Simplex Virus (HSV) Transcription: An overview

  title={Peculiarities of Herpes Simplex Virus (HSV) Transcription: An overview},
  author={J{\'u}lius Raj{\vc}{\'a}ni and Vojvodov{\'a} Andrea and Re{\vz}uchov{\'a} Ingeborg},
  journal={Virus Genes},
The herpes simplex virus (HSV) has a 152 kbp dsDNA encoding probably 84 proteins. The approximate number of ORFs is 94, from which seven are doubled. The most probable number of single copy ORFs is 84 after omitting the two latency associated transcripts (LAT)/ORFs and the putative UL27.5 ORF. The high gene number creates a “crowded” genome with several overlapping transcripts. The unique long (UL) segment has at least 10 interposed ORFs, the existence of which was not obvious at first sequence… Expand
Integrative functional genomics decodes herpes simplex virus 1
The complete viral transcriptome and translatome during lytic infection is unraveled with base-pair resolution by computational integration of multi-omics data and shows that key viral regulators and structural proteins possess NTEs, which initiate from non-canonical start codons and govern subcellular protein localization and packaging. Expand
Integrative functional genomics decodes herpes simplex virus 1
The complete viral transcriptome and translatome during lytic infection is unraveled by computational integration of multi-omics data and it is shown that NTEs with non-canonical start codons govern the subcellular protein localization and packaging of key viral regulators and structural proteins. Expand
Transcriptional Regulation of Latency-Associated Transcripts (LATs) of Herpes Simplex Viruses
Seven novel transcription factors and their corresponding transcription regulatory sequences of HSV LATs are discovered, suggesting they are potentially functional in regulating HSV gene transcription, including LAT transcription. Expand
Human CMV transcripts: an overview.
The human CMV genome consists of an approximately 230-kb dsDNA and is predicted to contain over 165 open reading frames and major alternately spliced mRNA species of HCMV originate primarily, but not exclusively, from the immediate early gene regions. Expand
Molecular characterization of the herpes simplex virus 1 (HSV-1) homologues, UL25 to UL30, in duck enteritis virus (DEV).
Phylogenetic trees of the putative proteins encoded by these seven genes showed that UL27, UL28, and UL30 had a close evolutionary relationship with the Mardivirus, however, the other four proteins exhibited close relationships with the Simplexvirus or Varicellovirus, indicating that the DEV clone-03 should be placed into a single cluster within the subfamily Alphaherpesvirinae. Expand
Searching for RNAP II on the Compact HSV-1 Genome
A collision model where the compact viral genome becomes overburdened by the high transcriptional activities at late time in HSV-1 infection, leading to arrested RNAP II complexes that then in turn, trigger a proteasome-mediated proteolysis of the polymerase to resolve stalled transcription is proposed. Expand
Transcriptional Analysis of Latent and Inducible Kaposi's Sarcoma-Associated Herpesvirus Transcripts in the K4 to K7 Region
In vitro analysis of KSHV-infected primary effusion lymphoma cell lines in the presence of 12-O-tetradecanoylphorbol-13-acetate and phosphonoformic acid suggests that one latent transcript is coterminal with the previously annotated K3 gene encoding an ubiquitin-ligase known to downregulate major histocompatibility complex class I expression. Expand
Long-Read Isoform Sequencing Reveals a Hidden Complexity of the Transcriptional Landscape of Herpes Simplex Virus Type 1
This work adds new insights for understanding the complexity of the pervasive transcriptional overlaps by suggesting that there is a crosstalk between adjacent and distal genes through interaction between their transcription apparatuses. Expand
Residues of the UL25 Protein of Herpes Simplex Virus That Are Required for Its Stable Interaction with Capsids
The first 27 amino acids are essential for capsid binding of UL25, while residues 26 to 39, which are highly conserved in the UL25 homologues of other alphaherpesviruses, were found to be critical for stable capsidbinding. Expand
In silico identification of putative promoter motifs of White Spot Syndrome Virus
The data may suggest the presence of two separate classes of late WSSV genes, one exploiting the cellular RNA polymerase II system for mRNA synthesis and the other generating messengers by a new virus-induced transcription mechanism. Expand


ed.), Genetic Maps
  • 1992
Conservative herpesvirus genes encoding proteins common for all the herpesvirus subfamilies HSV-1 ORF/gene block CMV ORF c-HV gene block EBV ORF HHV8 ORF Protein /function
  • J Virol 65,
  • 1991