Pectic acid lyases of Bacillus polymyxa.

  title={Pectic acid lyases of Bacillus polymyxa.},
  author={C W Nagel and Thomas M. Wilson},
  journal={Applied microbiology},
  volume={20 3},
Four enzymes were separated from an extracellular preparation of Bacillus polymyxa by carboxymethylcellulose column chromatography. The pH optima were 8.3 to 8.5, 8.7 to 8.9, 9.2 to 9.4, and 9.5 to 9.6. All of the enzymes required calcium ion for maximum activity, whereas strontium ion was only partially effective in stimulating activity. Cobalt was the only other cation tested which was effective in two of the enzymes. The lyases seem to attack a calcium salt-bridged substrate. K(m) and V(m… CONTINUE READING