Pax3 Inhibits Myogenic Differentiation of Cultured Myoblast Cells (*)

@article{Epstein1995Pax3IM,
  title={Pax3 Inhibits Myogenic Differentiation of Cultured Myoblast Cells (*)},
  author={Jonathan A. Epstein and Paula Y. P. Lam and Lisa I. Jepeal and Richard L. Maas and David N. Shapiro},
  journal={The Journal of Biological Chemistry},
  year={1995},
  volume={270},
  pages={11719 - 11722}
}
Pax3 is an evolutionarily conserved transcription factor expressed in the lateral dermomyotome, a region that gives rise to limb muscle progenitors. Mutations in Pax-3 account for the mouse mutant Splotch which develops without limb musculature. We demonstrate that Pax3 can inhibit myogenic differentiation of C2C12 myoblasts normally induced by exposure to low serum. Specific missense mutations that affect the DNA binding characteristics of the two distinct DNA binding domains of Pax3 abolish… 
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Examination of cell survival and gene expression in presomitic mesoderm, somites and neural tube of developing wild-type and Pax3 mutant (Splotch) mouse embryos establishes that Pax3 has complementary functions in MyoD activation and inhibition of apoptosis in the somitic mesderm and in repression of Pax7 during neural tube and somite development.
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Results suggest that Thr115 may play an important role in the regulation of MyoD function under conditions of high mitogenesis.
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In muscle progenitor cells, Pax3 and Pax7 function to maintain expression of myogenic regulatory factors, and promote population expansion, but are also required for myogenic differentiation to proceed.
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Mutation of Thr115 in MyoD positively regulates function in murine fibroblasts and human rhabdomyosarcoma cells.
  • L. N. Liu, P. Dias, P. Houghton
  • Biology
    Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
  • 1998
TLDR
A mutant MyoD with a single amino acid substitution that disrupts the PKC phosphorylation site (Thr115) within the conserved basic domain suggests that Thr115 may play an important role in the regulation of Myo D function under conditions of high mitogenic conditions.
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