Patterns of transformation intensity on flax hypocotyls inoculated with Agrobacterium tumefaciens

@article{Dong1991PatternsOT,
  title={Patterns of transformation intensity on flax hypocotyls inoculated with Agrobacterium tumefaciens},
  author={Jin-zhuo Dong and Alan McHughen},
  journal={Plant Cell Reports},
  year={1991},
  volume={10},
  pages={555-560}
}
Cellular transformation intensities on flax (Linum usitatissimum) hypocotyl explants using disarmed Agrobacterium tumefaciens were investigated through various preculture durations, cocultivation durations and removal of epidermis. The expression of an intron-containing β-glucuronidase (GUS) gene driven by CaMV 35S promoter served as a reporter for determination of transformed tissues on hypocotyls. The binary plasmid p35SGUSINT in octopine-type Agrobacterium strain GV2260 was used as the… CONTINUE READING
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