Patterns of protein adsorption in chromatographic particles visualized by optical microscopy.

@article{Stone2007PatternsOP,
  title={Patterns of protein adsorption in chromatographic particles visualized by optical microscopy.},
  author={Melani C Stone and Giorgio Carta},
  journal={Journal of chromatography. A},
  year={2007},
  volume={1160 1-2},
  pages={206-14}
}
A new method is presented to image transient patterns of protein adsorption in individual spherical chromatographic particles under strong binding conditions. The method takes advantage of the difference in refractive index between the protein-free and protein-saturated adsorbent matrix. When the particles are viewed with an ordinary microscope using white light illumination, the adsorption front appears as a bright ring that moves in time from the surface of the particle to its center… CONTINUE READING

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