Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA.

@article{Stiller2006PatternsON,
  title={Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA.},
  author={Mathias Stiller and Richard E. Green and Mark Ronan and Jan Fredrik Simons and Li-Lin Du and Wan-Zhong He and Michael Egholm and Jonathan M. Rothberg and Susan G. Keates and Nikolai D. Ovodov and E E Antipina and Gennady F. Baryshnikov and Yaroslav V. Kuzmin and Aleksandar Vasilevski and Gerald E Wuenschell and John S Termini and Michael Hofreiter and Viviane Jaenicke-Despr{\'e}s and Svant P{\"a}{\"a}bo},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2006},
  volume={103 37},
  pages={13578-84}
}
Whereas evolutionary inferences derived from present-day DNA sequences are by necessity indirect, ancient DNA sequences provide a direct view of past genetic variants. However, base lesions that accumulate in DNA over time may cause nucleotide misincorporations when ancient DNA sequences are replicated. By repeated amplifications of mitochondrial DNA sequences from a large number of ancient wolf remains, we show that C/G-to-T/A transitions are the predominant type of such misincorporations… CONTINUE READING

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