Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica.

@article{Wolk1976PathwayON,
  title={Pathway of nitrogen metabolism after fixation of 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica.},
  author={Coleman Peter Wolk and J O Thomas and Paul W. Shaffer and Sam M. Austin and Aaron Galonsky},
  journal={The Journal of biological chemistry},
  year={1976},
  volume={251 16},
  pages={5027-34}
}
Methods have been developed for identifying the pathway of assimilation of N2-derived nitrogen. The products of fixation of 13N-labeled nitrogen gas ([13N]N2), and the distribution of 13N within glutamine, were determined after short periods of labeling (approximately 1 to 120 s) and also in pulse-chase experiments. Ammonia, the amide nitrogen of glutamine, and the alpha-amino nitrogen of glutamate, in that order, were the first observed products of fixation of N2 by the cyanobacterium (blue… CONTINUE READING

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