Pathogenicity and growth of Metarhizium anisopliae stably transformed to benomyl resistance

  title={Pathogenicity and growth of Metarhizium anisopliae stably transformed to benomyl resistance},
  author={Mark S. Goettel and Raymond J. St. Leger and Srirama M. Bhairi and Mary Katherine Jung and Berl R Oakley and Donald Wilson Roberts and Richard C. Staples},
  journal={Current Genetics},
The insect pathogenic hyphomycete Metarhizium anisopliae was transformed to benomyl resistance using pBENA3, a plasmid containing the benA3 allele from Aspergillus nidulans. The transformation rate was 9 transformants/50 μg DNA/2×106 viable protoplasts. Southern hybridization analyses indicated that the plasmid integrated by nonhomologous recombination at multiple loci. The sites of integration differed among transformants. There was no evidence for autonomous plasmid replication in the… CONTINUE READING

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Anal Biochem 132:6-13

AP Feinberg, B Vogelstein
Acad Sci USA • 1983

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