Association of tuberculin-boosted antibody responses with pathology and cell-mediated immunity in cattle vaccinated with Mycobacterium bovis BCG and infected with M. bovis.
Description Attempts to control bovine tuberculosis in GB are hampered by lack of scientific knowledge in several key areas: (1). A true picture of the pathology, immunology and clinical pathology of reactor cattle; (2). The extent to which ongoing infection in herds is due to cattle to cattle spread being maintained by animals falsely testing negative to the skin test. By undertaking detailed pathological, clinicopathological, bacteriological and immunological examination of 200 reactor and 200 in contact nonreactor cattle, it will be possible to define many of the parameters critical for experimental and modelling studies. These include, lesion localisation, the extent of nasal shedding in the early stagers of disease and the role of concurrent infection as a covariate in pathogenesis. The detailed immunological profiling will assist in validating markers of early disease and pathology, which should help to detect animals in the early stages of infection. All results arising from the study will be closely integrated by using advanced systems of data capture, transfer and storage, and the experience gained should assist MAFF in other complex field studies, such as the current Krebs’ trial. Objective 01 31/03/2001 Develop intranet based method for data capture and transfer