Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity

@article{Sun2021PathogenER,
  title={Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity},
  author={Xinhua Sun and Dmitry Lapin and Joanna Marie Feehan and Sara Christina Stolze and Katharina Kramer and Joram A. Dongus and Jakub Rzemieniewski and Servane Blanvillain-Baufum{\'e} and Anne Harzen and Jaqueline Bautor and Paul Derbyshire and Frank L. H. Menke and Iris Finkemeier and Hirofumi Nakagami and Jonathan D. G. Jones and Jane E. Parker},
  journal={Nature Communications},
  year={2021},
  volume={12}
}
Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to detect pathogen effectors and activate local and systemic defence. NRG1 and ADR1 “helper” NLRs (RNLs) cooperate with enhanced disease susceptibility 1 (EDS1), senescence-associated gene 101 (SAG101) and phytoalexin-deficient 4 (PAD4) lipase-like proteins to mediate signalling from TIR domain NLR receptors (TNLs). The mechanism of RNL/EDS1 family protein cooperation is not understood. Here, we present… 
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Plant immune networks.
From plant immunity to crop disease resistance
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References

SHOWING 1-10 OF 53 REFERENCES
NRG1 functions downstream of EDS1 to regulate TIR-NLR-mediated plant immunity in Nicotiana benthamiana
TLDR
This study demonstrates that NRG1 is a key component that acts downstream of EDS1 to mediate various TNL signaling pathways, including Roq1 and RPP1-mediated HR, resistance to Xanthomonas and Pseudomonas, and XopQ-regulated transcriptional changes in N. benthamiana.
Differential regulation of TNL-mediated immune signaling by redundant helper CNLs.
TLDR
Through CRISPR/Cas9 gene editing methods, it is discovered that the tandemly repeated NRG1A andNRG1B are functionally redundant and operate downstream of TNLs with differential strengths, suggesting a cytosolic activation mechanism contributing to TNL-specific immunity.
The Nuclear Immune Receptor RPS4 Is Required for RRS1SLH1-Dependent Constitutive Defense Activation in Arabidopsis thaliana
TLDR
Analysis of RRS1SLH1 shed new light on mechanisms by which NB-LRR protein pairs activate defense signaling, or are held inactive in the absence of a pathogen effector.
Origins and Immunity Networking Functions of EDS1 Family Proteins.
TLDR
EDS1 family protein functions across seed plant lineages are considered in the context of networking with receptor and helper NLRs and downstream resistance machineries to explain their central role in biotic stress resilience.
An EDS1-SAG101 Complex Is Essential for TNL-Mediated Immunity in Nicotiana benthamiana.
TLDR
Analysis of EDS1 functions in the model Solanaceous plant Nicotiana benthamiana demonstrates the value of genetic resources in Nb, which will facilitate elucidation of E DS1 functions, and identifies a large surface essential for EDS 1-SAG101 immune functions that extends from the N-terminal lipase domains to the C-Terminal EDS2-PAD4 domains and might mediate interaction partner recruitment.
An EDS1 heterodimer signalling surface enforces timely reprogramming of immunity genes in Arabidopsis
TLDR
It is shown that the EP-domain surface of EDS1 heterodimers signals downstream of both receptor types and ensures a rapid transcriptional response in TIR-NLR immunity to bacteria.
Balanced Nuclear and Cytoplasmic Activities of EDS1 Are Required for a Complete Plant Innate Immune Response
TLDR
It is proposed that coordinated nuclear and cytoplasmic activities of EDS1 enable the plant to mount an appropriately balanced immune response to pathogen attack.
Two unequally redundant "helper" immune receptor families mediate Arabidopsis thaliana intracellular "sensor" immune receptor functions
TLDR
Together, the genetic data confirm that RNLs contribute to basal resistance, are fully required for TNL signaling, and can also support defense activation during CNL-mediated ETI.
Arabidopsis SENESCENCE-ASSOCIATED GENE101 Stabilizes and Signals within an ENHANCED DISEASE SUSCEPTIBILITY1 Complex in Plant Innate Immunityw⃞
TLDR
It is demonstrated by a combination of cell fractionation, coimmunoprecipitation, and fluorescence resonance energy transfer experiments the existence of an EDS1–SAG101 complex inside the nucleus that is molecularly and spatially distinct from EDS2–PAD4 associations in the nucleus and cytoplasm, suggesting that dynamic interactions of EDS 1 and its signaling partners in multiple cell compartments are important for plant defense signal relay.
Evolution of Plant NLRs: From Natural History to Precise Modifications.
TLDR
The evolution of NLRs is discussed, an overview of previous engineering attempts are given, and how to use evolutionary knowledge to advance future research in the generation of novel disease-recognition capabilities are proposed.
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