• Corpus ID: 54052797

Pathobiological characterisation of Photobacterium damselae subsp . piscicida isolated from cultured sole ( Solea senegalensis )

  title={Pathobiological characterisation of Photobacterium damselae subsp . piscicida isolated from cultured sole ( Solea senegalensis )},
  author={Beatriz Magari{\~n}os and Jes{\'u}s L. Romalde and - SoniaL{\'o}pez and Romalde and Miguel {\'A}ngel Mori{\~n}igo and Alicia E. Toranzo},
Characterization of several Spanish strains of Photobacterium damselae subsp. piscicida isolated from cultured sole (Solea senegalensis, Kaup) was performed to investigate their antigenic and genetic relatedness with the P. damselae subsp. piscicida strains isolated from other fish species throughout the world. In addition, the virulence capability of the sole isolates was also examined. Antigenic analysis using agglutination tests and the dot blot assay indicated a serological homogeneity… 

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Photobacterium damselae subsp. piscicida: an integrated view of a bacterial fish pathogen
  • J. Romalde
  • Environmental Science
    International microbiology : the official journal of the Spanish Society for Microbiology
  • 2002
The methods employed in the laboratory to study the causative agent of pasteurellosis are discussed, from the diverse procedures for the detection and isolation of the pathogen to the latest molecular studies that have allowed its correct taxonomic allocation.
Phenotypic, antigenic, and molecular characterization of Pasteurella piscicida strains isolated from fish
The taxonomic analysis revealed that, regardless of the geographic origin and source of isolation, all the strains exhibited the same biochemical and physiological characteristics, and a correlation between plasmid carriage and resistance to a specific antimicrobial agent cannot be established.
Application of AFLP for taxonomic and epidemiological studies of Photobacterium damselae subsp. piscicida.
AFLP proved to be a useful genotypic technique for epidemiological surveys of the pathogen, since it was able to discriminate between Mediterranean and Japanese Photobacterium damselae subsp.
Isolation and characterization of the causative agent of pasteurellosis, Photobacterium damsela ssp. piscicida, from sole, Solea senegalensis (Kaup)
The first description of pasteurellosis affecting sole, Solea senegalensis (Kaup), cultured in the South-west of Spain is reported and the sensitivity pattern to antimicrobials and the enzymatic activities of the bacterial extracellular products are described.
Pathogenic activities of live cells and extracellular products of the fish pathogen Pasteurella piscicida.
The pathobiological activities in vivo and in vitro of live cells and extracellular products (ECP) of eleven Pasteurella piscicida strains of different origin were examined and are important for the selection of strains in the development of effective polyvalent pasteurellosis vaccines containing both whole cells and ECP.
16S rRNA Gene Sequence Analysis ofPhotobacterium damselae and Nested PCR Method for Rapid Detection of the Causative Agent of Fish Pasteurellosis
The results indicate that the selective primers which were designed represent a powerful tool for sensitive and specific detection of fish pasteurellosis and allow detection of the pathogen in mixed plate cultures obtained from asymptomatic fish suspected to be carriers of P. piscicida.
Photobacterium damselae ssp. piscicida: detection by direct amplification of 16S rRNA gene sequences and genotypic variation as determined by amplified fragment length polymorphism (AFLP).
A PCR protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16S rRNA gene sequences was developed, and Amplified Fragment Length Polymorphism (AFLP) can be an important tool for epidemiological and taxonomic studies of this highly homogeneous genus.
Detection of transferable R plasmids in strains of the fish–pathogenic bacterium, Pasteurella piscicida
Five out of the 60 strains were resistant to CM, tetracycline (TC), kanamycin (KM), NF and sulphamonomethoxine (SA), and transferable R plasmids were detected in these drug–resistant strains.