Pathfinder selection: in situ isolation of novel antibodies.

@article{Osbourn1998PathfinderSI,
  title={Pathfinder selection: in situ isolation of novel antibodies.},
  author={J. Osbourn and E. Derbyshire and T. Vaughan and A. Field and K. S. Johnson},
  journal={Immunotechnology : an international journal of immunological engineering},
  year={1998},
  volume={3 4},
  pages={
          293-302
        }
}
BACKGROUND To devise a novel method for targeted recovery of binding molecules from phage libraries. OBJECTIVES To assess the potential of the novel technique to the selection of human antibodies to specific cell surface antigens in situ, including carcinoembryonic antigen (CEA), E- and P-selectins, and to the selection of novel antibodies which recognize immobilized purified antigen. STUDY DESIGN Recovery of these antibodies from a naive human scFv library was effected using a 'pathfinder… Expand
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