Patch culturing and transfer of human fetal retinal epithelium

Human retinal pigmented epithelium (RPE) can be cultured by removing small patches of this layer from the choroid of the fetal eye. Such RPE patches give rise to healthy, epithelioid monolayers in vitro within 1–2 weeks without contamination from retinal or choroidal cells. The viability and proliferative capacity of these cultures is independent of the… (More)