Patch-clamp recordings from white matter glia in thin longitudinal slices of adult rat spinal cord.

@article{Nashmi2002PatchclampRF,
  title={Patch-clamp recordings from white matter glia in thin longitudinal slices of adult rat spinal cord.},
  author={Raad Nashmi and Alexander A. Velumian and Induk Chung and Liang Zhang and Sandeep Kumar Agrawal and Michael G. Fehlings},
  journal={Journal of neuroscience methods},
  year={2002},
  volume={117 2},
  pages={159-66}
}
We developed a technique of whole cell patch-clamp recordings from white matter oligodendrocytes and astrocytes in 200-250 microm-thick horizontal slices of adult (>2 months, 240-260 g) rat thoracic spinal cord. The viability of the white matter, sectioned in Na(+)-free, low Ca(2+) media, and the function of axons were preserved for >8 h, as demonstrated by the propagation of TTX-sensitive compound action potentials (CAPs) and the sensitivity of their refractory period to K(+) channel blocker 4… CONTINUE READING

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